Adhesion and Proliferation of Mesenchymal Stem Cells on Plasma-Coated Biodegradable Nanofibers

Various biomedical applications of biodegradable nanofibers are a hot topic, as evidenced by the ever-increasing number publications in this field. However, as-prepared suffer from poor cell adhesion, so their surface is often modified. In work, active polymeric layers with different densities COOH groups 5.1 to 14.4% were successfully prepared Ar/CO2/C2H4 plasma polymerization. It has been shown that adhesion and proliferation mesenchymal stem cells (MSCs) seeded onto plasma-modified PCL controlled CO2:C2H4 ratio. At high ratio, well-defined network actin microfilaments observed MSCs. Nanofibers produced at low ratio showed very survival. There significantly fewer on surface, they had small spreading area, poorly developed filaments, there almost no stress fibrils. The maximum percentage proliferating was recorded 35:15 compared gaseous environments 25:20 20:25 (24.1 ± 1.5; 8.4 0.9, 4.1 0.4%, respectively). Interestingly, differences between untreated plasma-polymerized = (4.9 0.6 0.4, Thus, polymerization can be an excellent tool for regulating viability MSCs simply adjusting